Aim: The aim of our study was to estimate serum Amylase and serum Lipase levels and to establish a correlation between Lipase/Amylase Ratio in Acute Pancreatitis using lipase and Amylase as enzymatic markers. Study Design: A hospital based cross sectional study was conducted on patients attending the Out patient department of Medicine and those visiting the emergency of SGRRIM & HS Dehradun Uttarakhand for a period of 6 months from April 2017 to September 2017. A total of 130 cases (70 Male & 60 Female) and an equal no. of controls (130) (70 Male & 60 Female) in the age group 20-80 years were selected randomly for the study. Exclusion criteria was age less than 20 years & more than 80 years, acute trauma, Diabetes mellitus, dyslipidaemia, End stage Renal disease etc. Methodology: 5 ml blood was collected in sterile containers form all the 260 subjects and was analyzed for serum Amylase and serum Lipase on fully automated Biochemical Analyzer 5600 of Orthodiagnostics. Results: The mean values of Lipase levels were found to be much higher in male cases (834.83 ± 407.37 ± 47.70) as compared to male controls (97.34 ± 51.71 ± 5.98). Similarly, the mean values of serum Amylase were higher in male cases (233.73 ± 116.69 ± 13.66) than male controls (65.81 ± 24.63 ± 2.85). The Lipase/Amylase ration in male cases was (6.92 ± 3.16 ± 0.37), whereas in male controls it was (1.53 ± 0.71 ± 0.08). Thus the level of Lipase/Amylase ratio is higher in male cases than controls.