Background: Kangaroo mother Care (KMC) is a type of care for low birth weight (LBW) babies whereby the LBW baby is placed in an upright position against the mother’s chest, with early skin-to-skin contact between the mother and infant. Despite the apparent feasibility of KMC, currently, only a few low birth weight babies in low-income countries have access to this intervention. Therefore, present study aimed to assess the effectiveness of self instructional module (SIM) on knowledge regarding importance of KMC among staff nurses at Geetanjali & Pacific hospital, in Udaipur. Method: Quantitative Pre-experimental research design was selected to conduct study. Only 80 staff nurses were selected as samples based on exclusion and inclusion criteria through non-probability convenient sampling techniques. Results: The mean post-test knowledge score 25.71 (91.82%) was apparently higher than the mean pre-test knowledge score 12.3 (43.93%). The calculated “t” value of 19.47 was significantly higher than the table value 1.96 at 0.05 level of significance. This indicates that there was significant difference in pre test and post test knowledge score of respondents and SIM was effective in increasing the knowledge of the respondents regarding importance of KMC for LBW babies. There was a significant association between knowledge of staff nurses and demographic variables such as Age in years (χ2= 10.473), Work experience (χ2= 10.196). Conclusion: Self instructional module was effective in improving the level of knowledge of staff nurses regarding Kangaroo Mother Care (KMC). Keywords: Self Instructional Modules; Staff Nurses; Low Birth Weight Babies; Kangaroo Mother Care.