A pre-experimental design with pre-test and post-test without control group was undertaken at urban area in Latur. 40 adolescent girls selected through Nonprobability convenient sampling technique to assess the effectiveness of structured teaching program regarding knowledge and attitude on Breast self-examination among the adolescent girls. The overall mean knowledge score of adolescent, girls during pre- test was 6.24±3.58 (SD) which is 31.36% of the total mean score, whereas during post–test, it was 5.8±1.09 (SD) which is 87.30% of the total mean score. Depicting difference of 55.93% increase in mean percentage of score. It reveals that the structured teaching program was effective among adolescents’ girls. Reveals that adolescent girls had gained adequate knowledge. It is observed that during pre-test the adolescent girls had poor overall knowledge whereas it was good after the implementation of structured teaching program. Highly significant difference was found between pre-test and post-test knowledge score. The study shows breast self-examination and its health effects was effective to improve the knowledge & attitude of adolescent girls at urban areas, Latur.