A study to assess the effectiveness of video assisted teaching regarding knowledge of care of neonate undergoing phototherapy among the 3rd year GNM student of Aminaben M Gangat Memorial School of Nursing, Surat was conducted by the investigator, in partial fulfilment as a requirement for the degree of Basic B.Sc. (N) Veer Narmad South Gujarat University during year 2015-2016. Objectives of the Study: 1.To assess the knowledge regarding care of neonate undergoing phototherapy among students. 2. To determine effectiveness of video assisted teaching regarding care of neonate undergoing phototherapy. Methods: An evaluative research approach with preexperimental design was used for the study. The study was conducted in Aminaben M. Gangat Memorial School of Nursing rampura, Surat. The sample comprised of 30 students of the 3rd year GNM. Sample was selected by using purposive sampling technique. Data was collected using a self structured questioner. Data was analyzed using descriptive and inferential statistics. The results of this study showed that highest percentage (53.33%) of student were in the age of 20 years. Majority (60%) of the students have previous exposure related to topic. The findings of the study concluded that students had inadequate knowledge regarding care of neonate undergoing phototherapy. The Video assisted teaching on care of neonate undergoing phototherapy was highly effective in improving the knowledge of students regarding care of neonate undergoing phototherapy.