Background: Urinary tract infection (UTI) is among the common bacterial infections worldwide and approximately 80% are associated with a urinary catheter. Catheter-associated UTI (CAUTI) has been associated with increased mortality, morbidity, length of hospital stay and the best way of CAUTI prevention is to adhere to recommended guidelines on infection control in the use of a urinary catheter. Complications associated with CAUTI cause discomfort to the patient, prolonged hospital stay, and increased cost and mortality. Aim: The aim of this study is to assess the knowledge of nurses towards risk contributing to CAUTI and reduce the incidence of CAUTI. Methods: An interventional study was performed to analyze the prevention of Catheterassociated Urinary tract infection. A prospective questionnaire was developed specially for this study. The questions regarding the indication for catheterization and measures for prevention of CAUTI were taken as per Centers for Disease Control (CDC) guidelines. Results: The overall data for HAI was collected, out of which the incidences of CAUTI was high. Conclusion: The conclusion of this study is, the CAUTI incidence is reduced through QCC (Quality Control Circle), providing a useful reference for the prevention of CAUTI and the development of medical quality improvement activities. There was a statistically significant gain in the knowledge of the nurses regarding the prevention of the CAUTI.