Umbilical cord is the vital direct interlink between mother and fetus which is always depicted as the relationship of an emotional bonding of motherhood which is a beautiful experience for a women. When mother gives birth the blood remains in the placenta and umbilical cord is referred as cord blood. Cord blood is the blood which remains in the umbilical cord and placenta post-delivery. Cord blood banking is the process of collecting the cord blood and extracting and cryogenically freezing its stem cells and other cells of the immune system for potential future medical use. Cord blood banking is a once in a life time opportunity to save our baby and the cord blood stem cells for potential medical uses like malignancies, bone marrow failure disorders, inherited metabolic and immunological disorder. A community based descriptive survey design study was conducted to assess the level of knowledge and attitude regarding umbilical cord blood banking among antenatal mothers. Materials and methods: The research approach used for this study was quantitative research approach and descriptive cross sectional survey design was adopted. By using purposive sampling technique 100 subjects were selected from primary health centre during antenatal clinic. Out of 100 subjects, 63 (63%) had inadequate level of knowledge and 66 (66%) of the subjects had positive level of attitude. There was a positive correlation between knowledge and attitude between the subjects towards umbilical cord blood banking. There was statistically significant association (p< 0.005 level). between the knowledge and attitude with selected demographic variables as type of family, monthly income, previous knowledge on umbilical cord blood banking. Conclusion: It was evident that antenatal mothers had positive attitude towards umbilical cord blood banking and inadequate knowledge about the umbilical cord blood banking. After data collection with ,the information booklet, the researcher created awareness to all the antenatal mothers and motivated to do the umbilical cord blood banking for the welfare of their children and they got positive feedback as most of the mothers registered for cord blood banking. This study enlightens the knowledge of the parents about umbilical cord blood banking and generate a strong voice for the children future all around the world.