Calcium and vitamin D are widely used therapies for osteoporosis, Vitamin D is produced in response to the action of sunlight on skin. The Efficacy of use of calcium and Vitamin D in all post-menopausal women for the prevention of fracture in uncertain. To assess the knowledge of vitamin D and calcium is most important in menopausal women to prevent its complications. Methodology: A quantitative research design was used for this study. A cross sectional descriptive survey was used. A total no of 60 samples were by using the purposive sampling technique. The data was collected by using structured questionnaire developed by investigator. Results: The major findings of the study as out of 60 subjects, Majority 38 (63.3%) of the menopausal women’s had moderately adequate level of knowledge, 18 (30%) had inadequate level of knowledge and 4 (6.7%) menopausal women’s had adequate level of knowledge 4 (6.7%). There was statistically significant association (p < 0.001) between the level of knowledge with demographc variable as age, monthly income of family and p < 0.05 level as source of information about Vitamin D and calcium role in menopause has significant association. Conclusion: It was concluded that the knowledge of menopausal women regarding Vitamin D and calcium shows that the Majority of the menopausal women’s had moderate adequate level of knowledge. So the community health personal motivate the menopausal women to promote to take calcium and Vitamin D intake during menopausal period to prevent from osteoporosis and major other health problem