A stem cell is a cell with the unique ability to develop into specialized cell types in the body. In the future they may be used to replace cells and tissues that have been damaged or lost due to disease. The present study aim to assess the knowledge regarding stem cell collection and preservation among 75 women residing at urban areas of Ahmedabad city with a view to develop an information booklet. The research design adopted for this study was Non experimental descriptive research design and non-probability convenient sampling technique was used for data collection. The study was conducted in urban areas of different societies of Ahmedabad city. Data collection tool consist of semi structured knowledge questionnaire with multiple choice questions. Data was analyzed through descriptive and inferential statistics. The study revealed that 17.33% samples had good knowledge, 73.33% samples had average knowledge and 9.33% had poor knowledge. Mean score regarding stem cell collection and preservation was 22.73. Among demographic variable Age in years, Education of women, Occupation of women, Marital Status, No. of children, Monthly Income, Religion, Source of information, Have they ever stored their Stem cells, it was found that there was significant association between occupation of women and knowledge of women. Chi square value was 8.171 and calculated P value was 0.016 which was less than 0.05 which indicated that it was significant. After analysis the investigator developed and distributed an information booklet regarding stem cell collection and preservation. Keywords: Stem Cell Collection and Preservation; Umbilical cord blood; Cord blood Transplants.