A study was conducted to find out the effectiveness of structured teaching programme by comparing the levels of knowledge regarding bio medical waste management among nursing personnel. Pre experimental one group pre test and post test research design was used. By using simple random sampling technique, 300 nursing personnel were selected. The outcome of the study revealed that before Structure Teaching Programme 130 (43.33 %) of nurses had moderately adequate knowledge, it is interesting to know that none of them had inadequate knowledge after STP. Paired ‘t’ test findings revealed that nursing personnel had improved their knowledge after structured teaching programme. The association between socio-demographic variables and knowledge regarding biomedical waste management were significant at 0.05 level. The study findings concluded that majority of the nursing personnel improved their knowledge regarding bio-medical waste management after structure teachingprogramme.