Background: India is a developing country with large population of child bearing age group, majority from low socioeconomic status. Unaware of the possibilities of asymptomatic bacteriuria and its complications and often neglecting minor symptoms due to ignorance, lack of medical facilities; ultimately face antenatal, perinatal and postnatal complications. Aim/Objectives: This study aimed at uropathogens associated with asymptomatic urinary tract infection in antenatal patients. The objectives of the study were: To determine rate of Asymptomatic Bacteriuria (ASB) in antenatal patients. To identify the uropathogens causing Asymptomatic Bacteriuria and to find out their antimicrobial susceptibility pattern. Methods: Clean catch midstream urine specimens were collected from 300 women of any gestational age who attended the Department of Microbiology from April 2014- April 2015. Uncentrifuged urine samples were taken for wet mount preparation. Cultures were done using CLED agar at 37o C. Antimicrobial susceptibility test were assessed using CLSI guidelines on Mueller Hinton agar. Results: Out of 300 antenatal patients Asymptomatic bacteriuria was positive in 10% of Women. E. coli and Staphylococcus aureus were the most common pathogen isolated which were found to be sensitive to Ceftriaxone and Ciprofloxacin (100%, 100%) respectively. Conclusion: The major pathogen isolated among 30 positive cases of Asymptomatic Bacteriuria were E. coli and Coagulase negative staphylococcus. All isolates of E.coli and Coagulase negative staphylococcus were found to be highly sensitive with antimicrobial particularly Ceftriaxone and Ciprofloxacin. Hence, this study recommends for screening cases of Asymptomatic bacteriuria and timely management is necessary to prevent the complications associated with asymptomatic bacteriuria.