Pseudomonas aeruginosa is a notorious, opportunistic pathogen and has ability of biofilm formation making it possible to survive in extreme conditions. As antibiotics and disinfectants have shown limited activity, P. aeruginosa phage based treatments are proposed as a promising way for biofilm control. P. aeruginosa isolates and PB1 like phage particles were isolated from sewage samples collected from sewage treatment plants. PB1 like phage was featured with large burst size of 151PFU/cell, host inactivation in 6 hrs, 80% of host range and had icosahedral head with a diameter of 85X80nm and long non contractile tail of 135nm. PB1 like phages are suitable for lysis of broad range MDR Pseudomonas biofilms, which could be used in phage therapy.