Wound healing is an array of planned steps in a sequence which includes inflammation, proliferation and remodelling. Wound healing depends on growth factors, cytokines, interleukins, etc. There is no well established method that accelerates wound healing. In this article we report how we have managed a case of post traumatic amputation stump who had developed iatrogenic SVC thrombosis, using different regenerative methods like the autologous platelet rich plasma, insulin therapy, prolotherapy, low level laser therapy for augmenting in wound healing. We also successfully managed SVC thrombosis during the hospital course. In this article we also discuss about the role of prolotherapy in wound healing.