Ascorbic acid, also known as vitamin C, is an essential water soluble vitamin. Body needs vitamin C in order to form and maintain bones, blood vessels and skin, and its deficiency causes scurvy. Ascorbic acid has been widely used in chemical, pharmaceutical, cosmetic and food industry for the preparation of various preparations. Analysis of ascorbic acid is an important and inseparable part of industries involved in any form of ascorbic acid preparation. This review highlights recent methods for ascorbic acid analysis like spectrophotometric method, high pressure liquid chromatography (HPLC), etc. Former methods for ascorbic acid analysis are also presented such as Titrimetric, spectrophotometric as well as HPLC method along with different detection methods.

Keywords: Vitamin C; Assay; HPLC; Spectrophotometry.