Quantitative microscopy has strengthened conventional diagnostic scheme through better  understanding of microscopic features from clinical perspective. Towards this, analysis of pathological image analysis havegained immense significance among medical fraternity through visualization and quantitative evaluation of clinical features. Till date, pathological inspection of rodents’ blood is solely dependent on subjective assessment which usually leads to significant inter-observer variation in grading and subsequently resulting in late diagnosis of certain diseases. This paper aims at a systematic approach to morphologically characterize of five types of white blood cells, and its nuclei from light microscopic image of blood samples. Hence, cellular and nuclei based geometric features are computed and statistically analyzed with t- test to show the discriminating potentiality of the species. 

Keywords: Rattus Rattus; Blood Cells; Differential Leucocyte Count; Morphometry.