Tissue culture technology is used for selection and rapid multiplication. In India the micro propagation technique has gained momentum to reoccupy the monopoly in Gymnema tissue culture at global level. It is able to regenerate millions of copies to ensure in a decade of time with high yielding with shorter duration. There is a tremendous demand for natural anti diabetic agents, because it have reducing blood sugar and are used as an anti diabetic agent in Indian medicine, improvement of cultivation is carried out by the in-vitro culturing or tissue culture technique. In-vitro studies or micro propagation of Gymnema were conducted at Agricultural College & Research institute, Killikulam at 2001. The shoot tip and nodal segments were collected and used as explants. These explants of Gymnema were inoculated in MS medium (Murashige and Shoog, 1962) supplemented with Kinetin (2.5 mg/lit), NAA (1 mg/lit), and GA3 (2.0 mg/lit) gave the better results for callus induction and shoot formation.

Keywords: Gymnema; Tissue culture; Kinetin; NAA.