The aim of the study was to evaluate and compare the physical properties of Heat & Chemically activated Denture Base Resin with addition of fillers. A total of 120 specimens were prepared. For evaluation of transverse strength and surface hardness of both Denture Base Resins, 80 specimens were prepared using standard mold measuring 65 mm x 10 mm x 2.5 mm, consisting of control plus experimental groups reinforced with three different fillers (5 wt% ZrO2 nanoparticles, 5 wt% Al2O3 nanoparticles, 2.5 wt% glass fibers). For evaluation of water sorption, 40 specimens were prepared using standard mold measuring 50 mm x 0.5 mm, consisting of control plus experimental groups reinforced with three different fillers. Results: Both Denture Base Resins showed increase in transverse strength and surface hardness after addition of fillers. Highest transverse strength and surface hardness was found to be in Heat Cure and Cold Cure reinforced with ZrO2 Nano Particles> Glass fiber >Al2O3 Nano Particles whereas the results of water sorption were in accordance with ISO specification. Conclusion: Reinforcement of both Denture Base Resins by addition of fillers can inhibit undesirable physical changes resulting from oral fluids and can prevent denture fractures by enhancing its properties.