The study aimed to evaluate and compare the effect of different denture cleansers on the physical properties of heat cure and cold cure denture base resins. A total of 150 specimens were prepared, seventy five from each denture base resin using a standard mold measuring 50 mm x 1.5 mm. For disinfection, specimens were immersed in denture cleansers (sodium hypochlorite and sodium perborate) for different time intervals (60 days, 90 days and 180 days). Surface roughness, hardness, color stability, sorption and solubility were measured for each specimen after the immersion procedure. Results: Both denture base resins tested exhibited a change in physical properties to some degree in both the cleansers. Surface roughness, change in color and solubility increased with time in both denture base resins with both the cleansers. However, the maximum increase of surface roughness, change in color and solubility was seen in CC DBR with NaBO3n H2O i.e. 2.20 μm, 2.3 ΔE and 2.9 μg/mm3 respectively. Hardness decreased with time in both denture base resins with both the cleansers. However, the maximum decrease was seen in CC DBR with NaBO3nH2O (13.20 VHN). Sorption increased with time in both DBR with both the cleansers. However, the maximum increase was seen in HC DBR with NaOCl (28.52 μg/ mm3). Conclusion: Sodium hypochlorite showed the best results when compared with sodium perborate solution and HC DBR showed the best result when compared to CC DBR.