Aims and objectives: To evaluate efficacy of dexmedetomidine in the dose of 0.5 ug/kg as a single bolus dose in attenuating hemodynamic response to laryngoscopy and tracheal intubation in adult patients. Materials and Methods: We conducted a prospective, randomized, double-blind study, in which 60 patients scheduled for elective surgery under general anesthesia were enrolled for the study. Patients were randomly distributed in two groups (30 in each group). Group D received a bolus dose of 0.5 ug/kg dexmedetomidine group and Group C received 10 ml of normal saline (control group). Results: There was significant decrease in heart rate in Group D as compared to Group C from 1 minute after induction till 80 minutes (p < 0.05). There was significant decrease in SBP, DBP and MAP in Group D as compared to Group C from laryngoscopy till postextubation (p < 0.05). Complications like hypotension, hypertension, bradycardia, tachycardia, agitation and coughing was observed in 0%, 80%, 0%, 83.33%, 23.33% and 40% of Group C patients respectively while it was present in 10%, 0%, 10%, 0%, 0% and 13.33% of Group D patients respectively. Conclusion: Single bolus dose of dexmedetomidine 0.5 μg/kg prior to laryngoscopy and endotracheal intubation attenuates the airway reflexes and hemodynamic responses effectively during induction of anesthesia providing smooth intubation and provides adequate sedation and delays the need for analgesia in the postoperative period.