Background: Successful Cardio Pulmonary Resuscitation for a good patient outcome requires teamwork and appropriate knowledge amongst health professional is necessary. Uniform protocols can be put in place only if hospital personnel have sound knowledge of Basic Life Support, which is achievable with regular training alone. We conducted this study to assess the knowledge and attitude among medical faculties, interns and students regarding BLS at our institute. Methods: An observational study was conducted at a tertiary care hospital from Gujarat, India. A self-prepared questionnaire was distributed to the participants and based on their responses; the percentage of knowledge and attitude regarding BLS was assessed. Results: Total 230 participants (94 faculties/senior residents, 36 junior residents/medical officers and 100 students/interns) were included in the study. For the purpose of analysis the faculty/SRs were divided in two groups based on their involvement in emergency services. The mean score (out of 15) for Faculty/ SR (Emergency) was 7.3, Faculty/ SR (Non- emergency) was 6.5, JR/ Mo was 8.1 and Student/ Intern was 8.0., indicating that Faculty/ SR (Non-emergency) scored significantly lower. Only one participant scored between 91-100%, five between 81-90%, 20 (8.7%) between 71-80%, 89 (38.69%) scored 51-70% and 115 (50%) scored less than 50%. Trained participants scored better versus those who were untrained (p = 0.0012). Years of clinical experience did not affect the knowledge scores (p = 0.3905). Conclusion: Overall knowledge of the doctors and students of our Institute was not satisfactory which warrants an Institutional policy for regular BLS training.