The present study was aimed to screen the presence of gastrointestinal endoparasites in Murrah buffaloes calves reared in the Buffalo Research Station (BRS), Venkatarammannagudem of Andhra Pradesh, India. A total of 44 dung samples were collected during the month of January 2021, from calves of different age groups ranging from 15 days to one year. Microscopic screening of the dung samples revealed presence of Buxtonella sulcata cysts and trophozoite forms in higher number and few helminthic infections of Toxocara vitulorum and Strongyle eggs. An overall incidence of the gastrointestinal parasites was found to be 43.18%, among which the incidence of Buxtonella sulcata was higher (36.36%) when compared to other endoparasites (6.81%). From the above study, it could be summarised that Buxtonella sulcata is a common inhabitant of gastrointestinal tract of buffaloes and responsible for diarrhoea in association with other helminths or mixed infections.