Objectives: An opinion regarding different teaching, learning and assessment techniques of gross anatomy taken from 147 first MBBS students at MGM Medical College and Hospital, Aurangabad, Maharashtra with the help of specially designed questionnaire. Materials and Methods: Cross sectional study was conducted in the Department of Anatomy MGM Medical College, Aurangabad, Maharashtra with the help of predesigned questionnaire to the interest of 1st MBBS students batch 2018–19 in different teaching, learning and assessment techniques of gross anatomy. Results: Out of total, 50.34% of them were male and all were between the age group of 18-22 years. Most of the students were from State Board (63.95%). 37.50% students preferred small group teaching. 54.91% preferred dissection hall teaching for practical. Textbook is preferred as a best source of study material by majority of students (45%). Most of the students consider LCD as the best teaching methodology. 61.11% students believe that multimedia teaching relies on scientific contents is not boring and does not cause lack of attention. According to majority of students (61.64%), multimedia teaching is affected by teaching method, skill of faculty and ability to imagine relationship. 51.68% students consider part completion test as the best assessment technique to measure knowledge in theory. Conclusion: Anatomy forms backbone of medical education for 1st MBBS students. This study assesses effectiveness of modern method versus old chalkboard method of teaching gross anatomy. Such studies give us an idea of our area of deficiencies and strengths of teaching methods which help in bringing corrective measures in future. Students welcomed PBL, horizontal and vertical integration and e-learning. Students preferred LCD (Lecture cum demonstration) over lecture with chalk and board. Surprisingly students preferred text book over teacher’s notes. Students are interested in multimedia teaching. Students favoured part completion test over weekly tests.