Neuroanatomists Murray Barr and Ewart Bertram in 1949 provided first study on sex chromatin, later named as Barr body. This led to a theory proposed by Mary F. Lyon in 1961 called as “Lyon hypothesis”. Since then the Barr body from buccal smear is used as a sex determination tool. However, with advent of modern molecular methodologies it is labeled as a useless tool by some investigators. Authors of this article wish to stress the importance of Barr body examination as a low cost effective tool.

Keywords: Sex Chromatin; Barr Body; Amenorrhea; Inguinal Hernia; Turner Syndrome.