The combination of HPLC with Mass Spectrometry (HPLC-MS) or Tandem Mass Spectrometry (HPLC-MS/ MS) is one of the most advanced and widely used technique for both qualitative as well as quantitative analysis of various diverse types of compounds including drugs, pesticides, micronutrients as well as naturally occurring toxic substances. This leads to the fact that many of the practitioners should be well versed with this technique. This review paper focusses on introduction, instrumentation as well as precautions that should be taken for using it. A wide variety of applications are also discussed along with advancements for making it time as well as cost-efficient.

Keywords: Mass Analyzer; Ionization Technique; MS Detector etc.