A number of benzodiazepine drugs viz. Diazepam, Flunitrazepam, Lorazepam, and Oxazepam, etc. have been used in the commission of such drugs facilitated sexual assault crimes and date rape case. Literature assessment reveals that only a few analytical methods are reported for the estimation of OXZ. Oxazepam drug was effectively extracted using micellar solution of Brij-58 (3mM) / Ethanol (1:1) solution. Limit of detection (LOD) and Limit of quantification (LOQ) are 0.6 ng/mL and 2.26 ng/mL of UV-Vis Spectrophotometric technique respectively. The linearity range (5-25ng/mL) for Oxazepam was obtained best with Coefficient of determination (R2=0.9998) and its wavelength of detection was 285 nm. A novel, safe and sensitive spectrophotometric method for estimation of Oxazepam drug in complex matrices viz. human blood, milk and urine have been developed and validated according to ICH guideline. This method can be scaled up in forensic science and toxicological laboratories.

Key words: Extraction; Oxazepam; surfactant; Complex matrices; Spectrophotometric techniq