Application of DNA fingerprinting in crime investigation is a routine process across the globe. Next Generation Sequencing (NGS) is the recent advancement of the technology. Capillary electrophoresis is routinely used in most of the forensic laboratory globally. In this study, an infant is disposed of in a common toilet and postmortem showed that the infant was male of an intra-uterine period of about 30 ± 2 weeks. The birth of the infant was premature and he did not respire after birth. Since no evidence was available to connect the mother with the unknown neonate. Scientific evidence like DNA test with autosomal STR markers was the last tool to investigate the situation. DNA profiling of infant and the suspected mother was prepared by using AmpFℓSTR® Identifiler® kit having 16 autosomal markers. Half set of alleles of infant’s DNA profile was accounted in the female DNA profile obtained from suspected mother. DNA profiling of both the infant and the suspected mother proved that she is the biological mother of the infant.

Key words: Maternity; DNA Fingerprinting; STRs; AmpFℓSTR® Identifiler® kit.