Genetic polymorphism indices and forensic parameters at 22 autosomal short tandem repeat (STR) loci were evaluated in 248 unrelated individuals of north-western Indian state Rajasthan. In total, 258 alleles were observed with an average of 11.72 alleles per locus. The most polymorphic and discriminative locus was Penta E with a value of 0.904 and 0.913 respectively. The combined power of exclusion and the combined power of discrimination were found to be 0.999999999 and 1 respectively. Additionally, the genetic relationship of the studied population with the reported Indian as well as global populations was investigated. The studied population showed genetic affinity with the previously reported population of Rajasthan and geographical close population’s i.e. populations of Uttar Pradesh, Madhya Pradesh and Jharkhand. All the STR loci were found polymorphic, and the select panel of STRs was found suitable for population genetic studies and forensic analysis.

Keywords: Power plex fusion 5C system; Rajasthan; Genetic diversity; Polymorphism; STRs