Aim: Cholera is endemic in many parts of India and a major public health problem. The present study was carried out with the aims to understand biochemical characters and drug resistance pattern of Vibrio cholera isolates obtained at a backward region of Gulbarga district. Karnataka. Study Design: Descriptive retrospective study was carried out to study V. cholerae isolates from 300 faecal specimens of patients with acute gastroenteritis. Place and Duration of Study: The study was conducted during 2006-2008 at Department of Microbiology, Gulbarga University, and Gulbarga. Karnataka. Methods: A total 28 isolates of V. cholerae were included in the study. V. cholerae was identified by standard microbiological procedures. Biochemical characters and antibiogram analysis, followed by biochemical characters. Antibiotic sensitivity testing was performed by Kirby-Bauer disc diffusion method. Results: V. cholerae strains were isolated from 28 faecal specimens. Male: Female ratio was 1:1.5 and high incidence was seen in 0-10 age group (35.71%). Maximal occurrence in monsoon season was recorded. All the isolates were resistant to co-trimoxazole, nalidixic acid and ampicillin. However maximum sensitivity was observed to norfloxacin (71.42%) following by gentamycin (67.85%) and chloramphenicol (28.57%). Conclusion: A continuous surveillance for V. cholerae is required with respect to changing epidemiology and emergence of antibiotic resistance strains. the source and spread of infection should be investigated to decide the proper management strategies. Additionally, quality of water and status of sanitation should be monitored.