Background: Calcium (Ca) and phosphorus (P) are indispensable micronutrients to human nutrition for their infinite role in structural and transduction processes. They are the molecules of central attraction due to their role in health and predicting the severity of different diseases. Aims and objectives: Considering their importance in health and disease, study was aimed to document the normal serum levels of calcium and phosphorus in random population who visited the hospital for consultation during March-May 2021. Methods: A total of 711 subjects were recruited for the analysis of serum Ca and P. samples were analysed on fully automated analyser (Beckman Coulter, AU5800). Both Ca and P levels were measured by kinetic method. Results: The median age of the participants was 50 years. The overall median serum Ca and P levels recorded in the study population were as 8.9(Interquartile range [IQR=1.40]) and 3.4(Interquartile range [IQR]=1.18). About 4.5% of the study population was deficient in both Ca and P. Females had higher median calcium levels than males (p-value<0.05). A statistically significant association was observed between Ca and different age groups:-<20y,20-40 (P-value<0.003) and 20-40y,>40y40 (p-value<0.001). Further, a weak negative relationship was observed between Ca and Age (r=-0.118, p-value<0.002). Both Ca and P levels were low in 32(4.5%) patients(p-value<0.004). Conclusion: Our findings revealed, that our population has calcium and phosphorus levels within the normal reference range, but presence of low Ca and P levels in the small portion of our population may be helpful in efficacious management of serious illnesses including several time and money consuming diseases in future.