Background: Thalassemia is one of the major hemoglobinopathies among the population all around the world. Beta thalassemia major results in severe anemia which needs regular repeated blood transfusion, which leads to iron over load in the body which damages the liver, kidney and other. HIV, Hepatitis B and Hepatitis C are common among multiple blood transfusion patients. So to know the severity of the patient, we retrospectively & prospectively correlated transfusion transmitted infection, liver function test and serum ferritin with multiple blood transfused thalassemic patients. Methods: Total 200 subjects were studied. We examined all patients who are clinically and Hematological suspicion of thalassemia and patients diagnosed to have halassemia based on High Performance Liquid Chromatography (HPLC) over a period of 2 years 3 months. The patients groups were evaluated according to the clinicohematological presentation and HPLC study. Results: Mean value of serum ferritin in thalassemia major group was found to be significantly increased (4103.21 ± 2786.9 ng/ml). In thalassemia major group, out of 78 cases 4 cases found reactive for HBV and 9 cases found reactive for HCV. Conclusion: There is significantly higher seroprevalence of both hepatitis B and C markers was observed among the multi-transfused thalassaemic children. Blood screening has significantly reduced the risk of transfusion related hepatitis B and C. However, the risk is still there. So, the health care providers who are involved in the management and blood transfusion of thalassaemic patients should be more aware of this problem.

Keywords: Performance Liquid Chromatography (HPLC); Beta Thalassemia; Serum Ferritin; Transfusion Transmitted Infection (TTI).