Introduction: The Nephrotic syndrome aptly termed “the syndrome of Proteinuria” by the lord platt has stimulated provoked and puzzled doctors for years or more. The characteristic toad face, be if “Caucasian Negriod” or “ Mongoloid” is so pathognomic that together with bloated belly, legs and genitalia it has been recognized as a spectacular and specific since the time interest in thedisease of children began. Aims and Objectives:To Study Urinary Protein Pattern Using Polyacrylamide Gel Disc Electrophoresis in Nephrotic Syndrome. Meterials and Methods: This is a retrospective study. The study was carried out in department of ACPM Medical College, Dhule. Diagnosed cases of Nephrotic syndrome of different age and sex admitted to hospitals during one year were included in study. Clinical diagnosis of Nephrotic syndrome was recommended by international study of kidney disease for diagnosis used were as follows (more than 3 gm in 24 hrs/1.73 m2 /more than 40 mg/hr/m2 ) (serum cholesterol more than 200 mg/dl) (hypoalbuminaemia-serum albumin less than 2.5 g/dl. Result: Maximum number of patient 54(64.28%) were-observed in 0-10 years age group. Of this 54 patients, 28(51.85%) were male and 26 (48.14%) were female. So male female ratio is 1.07:1 The prevalence of nephrotic syndrome decreases as age advance. Male female ratio is 1.5:1. Oedema was the most common presenting symptom and it was observed in all patients (100%). The next common presenting symptom was fever which was observed in 47(55.95%) patients. Associated diseases were found in 23 cases of nephrotic syndrome the most common disorder associated with nephrotic syndrome was glomerulonephritis which was observed in 15(65.22%) patients. The next common associated systemic disorder was tuberculosis which was observed in 5 cases (21.74%) Commonest pattern observed was presence of albumin and transferrin (II) among 36 patient (42.85%) followed by presence of albumin, transferrin, post albumin and post transferrin (IV) in 15 patients (17.85%). Albumin, transferrin and post albumin (III) was observed in 9 patients (10.71%) and only albumin (I) in seven patient (8.33%). Whereas presence of all proteins (VI) was noted in 3 patients (3.57%) and presence of albumin, transferrin, post albumin and post transferrin and 7S (V) in 14 patients (16.66%). Pattern ‘I’ protein in range of 2 -2.9 g/24hours, in thepattern II protein in range of 2-2 g/24 hours . Type ‘III’ protein levels of 2-2.9gm/24 hours- pattern ‘IV’ -3-3.9gm/24 hours. Pattern ‘V’- 3-3.9 gm/24 hours . Pattern VIin 3-3.9. Conclusion: Polyacrylamide Gel Disc Electrophoresis in Nephrotic Syndrome is useful of quantification and identification of the nephrotic syndrome.

Keywords: Nephrotic Syndrome; Polyacrylamide Gel Disc Electrophoresis.