AbstractFoods rich in antioxidants have gained much attention due to their health promoting activities. These foods are also referred as a neutraceuticals as they prevent many of degenerative diseases like diabetes mellitus, cancer, blood pressure and heart diseases. Garden cress seed have been used in India since ancient time. It possesses numerous health benefits such as antianaemic effect, Antimicrobial Activity, Antidiabetic and Anti-oxidant effect. In the present study, Garden cress seed was evaluated for its in vitro antioxidant and antidiabetic activities. Antioxidants activities of Garden cress seed was studies by DPPH and ABTS method. Whole seed showed 22.63% DPPH radical inhibition and 13.78% ABTS radical inhibition. Antidiabetic activity was studies by using Non enzymatic Glycosylation of hemoglobin (NEGH) and a amylase inhibition activity. Non enzymatic Glycosylation of hemoglobin inhibition was 71.42% and a amylase inhibition was 66.53%. Results suggest potential health benefiting activities of Garden cress seed. These benefits must be utilized by incorporating seed in daily diet.