The use of biological evidence for identification of an individual is relatively recent development. Dental identification has long been considered a reliable method when other methods fail because of critical body conditions or unavailability of body parts. Tooth pulp is encased in a hard tissue where it may be protected from detrimental effects of impact, trauma, and heat. Casperson et al was the first to develop a technique using pulpal tissue stained with quinacrine mustard, specific for Y chromosome to determine sex of an individual. This article describes the use of dental pulp in sex determination both antemortem and postmortem.