Backgrounds: Cancer is a disease which is characterized by uncontrolled cell division. There are several markers depicting the onset of cancer, out of which expression of the regulatory proteins is most significant and accurate. The p53 is a most targeted gene that regulates the cell cycle and maintains the integrity of DNA. Aim & objectives: The objective of this study was to evaluate the expression of p53, tumor suppression gene level in different types of cancer. Materials and Method: The study was conducted at Lavanya Ayurvedic Hospital of Cancer & AIDS & Research Centre, Lucknow. The drug used in this study was a bio-mineral formulation prepared by its own manufacturing unit. After approval of Institutional Ethical committee, the blood samples were collected from the patients after written consent. The test was performed by solid phase sandwich enzyme linked immuno-sorbent assay (ELISA) as per standard protocol of kit. Results: After one month treatment with drug the post treatment values of p53 of different malignancies were significantly higher than the pre treatment status. Conclusions: In this pilot study it is concluded that after treatment with the bio-mineral drug the expression pattern of tumor suppression gene, p53 enhances which indicates the effectiveness of drug in different types of cancers. 

Keywords: Bio mineral formulation; p53 Tumor suppression gene.