Abstract Staphylococcus aureus is one of the most common causes of nosocomial or community-based infections that were resistant to â-lactam antibiotics due to production of ß-lactamase and presence of mecA gene. Most laboratories use disk diffusion method and MIC for detection of methicillin resistance in S. aureus. The latex agglutination test is an alternative to PCR for rapid detection of mecA gene. The aim of the study is to detect and differentiate BORSA (Borderline Oxacillin Resistant Staphylococcus aureus) from MRSA (Methicillin Resistant Staphylococcus aureus) and to compare MRSA latex agglutination test with standard susceptibility testing methods viz., oxacillin agar screen, Cefoxitin disc diffusion test and MIC.