Introduction: Tuberculosis is major communicable diseases, scattered thought the world, affected by all age group. World health organization is considered is a major health problem so that it is integrated with other health agencies to provide holistic care. The treatment mainly included early diagnosis and treatment like provide essential drug to tuberculosis patient and also trained health worker or other designated individual provides the prescribed Tb drugs and watch the patient swallow every dose. Some studies shows that 85–90% of patients receiving DOT complete therapy compared to 61% for those who are self-administered therapy. DOTS help patient’s completed TB therapy as soon as possible without unnecessary gaps. The aim of this study is to understand the knowledge and attitude of tuberculosis patient’s regarding DOTS. Method: This study was conducted in DOTS center Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow. Convenient sampling technique was used to recruit 30 samples. Result: The study shows that knowledge score 50 of clients receiving DOTS therapy; that is 50% had inadequate knowledge, 40% had moderate knowledge and 10% had adequate knowledge, similarly shows the attitude scores of clients receiving DOTS therapy; that is 3% had poor attitude, 87% had moderate attitude and 10% had good attitude. Conclusion: The study concluded that tuberculosis patients should change their health attitude and practice towards DOTS. 

Keywords: Knowledge; Attitude; DOTS; Therapy; Center