Background: The corona virus disease 2019 (COVID-19) emerged in Wuhan, China at the end of 2019. Since then, it has spread to 200 countries and has been declared a global pandemic by the World Health Organization (WHO). To date, there are more than 2.3 million positive COVID-19 cases recorded with at least 150,000 deaths globally. Aim: The overall aim of the study is to determine the knowledge of Covid -19 among nursing students in selected nursing colleges of Navi Mumbai and to assess the association between knowledge score and selected demographic variables among nursing students in selected nursing colleges of Navi Mumbai. Method: Descriptive survey approach with a typical descriptive design was adopted in the present study. Convenience sampling technique was used for the selection of the total 40 online samples. A self develop online knowledge questionnaire was used to assess the knowledge of Covid -19 among nursing students by using Google forms. Reliability of the knowledge questionnaire was checked by using split-half method and Spearman brown coefficient and the r = 0.9489. Prior permission from the concern authority of selected colleges was taken. The main study was conducted in Bharati Vidyapeeth Deemed to be University College of Nursing. After data collection the self-instruction module on information on Covid -19 was distributed to nursing students via mails to improve their knowledge. Result: Result revels that 37.5% of the nursing students had moderate level of knowledge and 62.5% had adequate level of knowledge. With the help of self instructional module students will improve the knowledge. Samples consist of 15% males and 85% females.17.5% were in 17-18yrs, 47.5% were in 18-19yrs and 30% were in 19-20yrs, 5% were in above 20yrs. Regarding background knowledge of COVID 19, 75% of the samples has knowledge on COVID. 75% of samples got knowledge from news channel, 10% samples got knowledge from doctors and 10% samples got knowledge from other sources. Conclusion: The overall findings of the study revealed that majority nursing students had moderate and inadequate knowledge regarding COVID-19. Hence, it is concluded that further improvement of knowledge on COVID-19 is needed. Keywords: Knowledge; Assess; Covid-19; Self-Instruction Module.