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RFP Journal of Biochemistry and Biophysics

Volume  3, Issue 1, Jan-June 2018, Pages 8-11
 

Original Article

Single Step Purification of Soybean Isoflavones Employing Silica Gel Adsorption Chromatography

MC Lakshmi1, MC Madhusudhan2, HS Prakash3, KSMS Raghavarao4

1Oscar House, La Pouquelaye, St Helier, JE13ZD, United Kingdom 2,3DOS in Biotechnology, Manasagangothri, University of Mysore, Mysuru 570006, India. 4Department of Food Engineering, CSIR-Central Food Technological Research Institute (CFTRI), Mysuru 570 020, India.

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Abstract

The extraction and purification of isoflavones from defatted soy flour (DSF) are attempted by leaching and adsorption chromatography. The isoflavones were extracted in methanol and processed employing silica gel column chromatography. After silica gel column chromatography 0.4 mg/ml of isoflavones content (3.9 mg/g DSF) was obtained which has 16.8 mg of total isoflavones. The process helps to the recovery of the isoflavones along with the removal of contaminants up to 90% which confirmed through HPLC.

Keywords: Concentration; Methanol; Isoflavones; Soybean; Column Chromatography.

 


Corresponding Author : MC Madhusudhan, DOS in Biotechnology, Manasagangothri, University of Mysore, Mysuru 570006, India