Background: Ambulance services are important part of health system in dealing with the emergencies and preventing mortality. The point of care testing of hypoglycaemia helps physicians with rapid results by using commonly ordered tests. This study was mainly taken up to study the role of POCT in diagnosis and providing immediate treatment for hypoglycaemia. Materials and Methods: A prospective study was undertaken in Ambulance services of the emergency department of Basaveshwara Medical College and Hospital, Chitradurga between March 2015 to July, 2019. The patients were subjected for a diagnostic work up by using point of care testing using GRBS. Results: Majority of the patients belonged to 51–60 years of age group, males sex and had diabetes since 11–15 years. The sensitivity of the point of care testing of hypoglycaemia was 78.03% and specificity was 90.21%. The inter
test agreement as indicated by Kappa statistics was 0.69 which is deemed to be good. Conclusion: The point of care testing provides the diagnosis at an earliest point of time and thus helps in early treatment and prevention of mortality.