Background: Preeclampsia affects 2-8% of pregnancies and is a major cause of maternal and perinatal morbidity and mortality. Without intervention, the mother is at substantial risk for seizures (eclampsia), renal and liver failure, pulmonary edema, stroke, and death. Preeclampsia is also recognized as a major risk factor for cardiovascular disease later in life for both the woman and her child. HCG has many important functions during pregnancy including promotion of progesterone production, implantation and decidualization, angiogenesis, cytotrophoblast differentiation and immune cell regulation. AFP serum levels during pregnancy also have been used as an ancillary aid in the diagnosis of placental abnormalities, fetal death, growth restriction/retardation, and preterm labor. Aims and Objective: 1. To evaluate maternal serum mid-trimester human chorionic gonadotropin (HCG) and alfa-fetoprotein levels (AFP). 2. To look for correlation between maternal serum mid-trimester human chorionic gonadotropin and alpha-fetoprotein levels with development of preeclampsia. Material and Methods: Study Design: Descriptive and observational prospective study. Study Area: The study was conducted in department of obstetrics and Gynaecology, at Tertiary care center. Statistical Analysis : Data was analyzed using SPSS Ver 23. Frequencies, Descriptive, chi square test were done. Results: The mean AFP who developed severe preeclampsia was 58.84 ± 7.01 ng/ml compared to 42.85 ± 15.27 ng/ml who developed mild preeclampsia compared to 32.42 ± 8.17ng/ml who remained unaffected during study (p < 0.05). The mean β HCG who developed severe preeclampsia was 58510.00 ± 9065.68 mIU /ml compared to 42398.13 ± 13615.98 mIU /ml who developed mild preeclampsia compared to 29154.24 ± 12330.26 mIU /ml who remained unaffected during study (p <0.05). Conculsion: Elevated levels of maternal serum AFP & β hCG measured between 14 – 24 weeks of gestation have significant positive correlation with pre-eclampsia & adverse obstetric outcome. Second trimester markers (AFP & β hCG) may be used as predictor of preeclampsia.

Keywords: Preeclampsia, Alfa feto protine (AFP), Human chorionic gonadotropin (HCG)