Nosocomial infections represents a universally serious health problem and a major concern for the safety of both patients and the health care providers. NIs has significant consequences on patients, their families, and the community as a whole. Nurses’ are responsible for providing medications, dressing, sterilization, and disinfection. They are involved in more contact with patients than other health care workers (HCWs). Accordingly, they should be aware of how to prevent transmission of NIs and be knowledgeable of its potential riskto patients, otherstaff, and asvisitors. A descriptive study design was used in this study. A non-probability (purposive) sampling technique was used to select the study participants. A total of 85 student nurses of Final year of GNM, B.Sc. Nursing participated in this study and responded to the study questionnaire. The 30 items related to knowledge were assessed and 1 Score was given for each correct response. A score of less than half of the total was grouped as poor, 51–80% fair, and 81%and above was grouped as good. The outcome was decided as significance at the level of 0.05. The results of the current study showed that the majority (87%) of the participants had a fair level of mindfulness on the different Nosocomial infection preventive measures. However, poor level of knowledge was showed by 9%, and only 4% had good level of knowledge on the different Nosocomial Infections’ preventive measures. The results highlighted the necessity to implement an in-service training on infection control measures with more focusing on safe injection awareness and safe linen handling because student nurses had poor knowledge in these two aspects.