Background: The glycopeptide vancomycin was considered to be the best alternative for the treatment of multi drug resistant MRSA. However, there are increasing numbers of reports indicating the emergence of vancomycinresistant Staphylococcus aureus (VRSA) strains exhibiting two different resistance mechanisms. The emergence and spread of resistance to vancomycin is a threat to the already challenging therapy of MRSA. Materials and Methods: The present study was carried out to find out the presence of VISA and VRSA in the tertiary hospital. A total 570 staphylococcus aureus isolates consisting of 340 MRSA and 230 MSSA were isolated from different clinical specimens from various outpatient departments and wards using the standard techniques. All MRSA isolates were subjected to disc diffusion testing and MIC testing against vancomycin. Result and Discussion: Out of the 340 MRSA isolates, 270 isolates were Vancomycin sensitive Staphylococcus aureus (VSSA) (MIC: 0.5-2µg/ml), 70 isolates were Vancomycin intermediate Staphylococcus aureus (VISA) (MIC: 4-8µg/ml) and none were Vancomycin resistant Staphylococcus aureus (VRSA) (MIC: >16µg/l). The present study reveals for the first-time emergence of VISA/VRSA from this part of Karnataka, India. Keywords: Staphylococcus Aureus; MRSA; Vancomycin; VRSA; VISA.