Background: Diabetes mellitus is a metabolic disorder in which there is increase in the levels of blood glucose because of insulin deficiency. Diabetic foot ulceration and infections are major medical, social, economical problem and it is the leading cause of morbidity and mortality in developing countries like India. The present study is an attempt made to know the aerobic bacteriological profile of diabetic foot ulcers. Material and methods: A total of 100 patients with diabetic ulcers admitted in surgical wards were studied. Pus was collected using two swabs from each patient one for staining and the other for aerobic culture. The organisms isolated were identified using standard techniques. Antimicrobial susceptibility of the bacterial isolates was done by Kirby-Bauer disc diffusion method. Result: Polymicrobial etiology was observed in 59% and monomicrobial etiology in 41%. A total of 165 organisms were isolated. Most common isolates were staphylococcus aureus 38 (23.03%), followed by Klebsiella spp 34 (20.6%), Pseudomonas aeruginosa 28 (16.96%), Escherichia.coli 26 (15.75%), Proteus spp 23 (13.93%), Enterococcus faecalis 8 (4.84%), Citrobacter spp 4 (2.42%) and Staphylococcus epidermidis 4(2.42%). Most sensitive antibiotics were Imipenem, amikacin, ciprofloxacin and Gentamicin. Conclusion: Culture and sensitivity from the wound plays an important role in prescribing the appropriate antibiotic at the time of admission itself rather than starting empirical treatment. Thus proper antibiotics policy and measures to restrict the indiscriminate use of antibiotics should be taken to minimize the emergence of drug resistant pathogens. Keywords: Diabetic Foot Ulcers; Staphylococcus aureus; Klebsiellaspp; Citrobacter Spp; Polymicrobial etiology.