AbstractIntroduction: Group B streptococcus (GBS) is an important cause of illness in newborns, pregnant women as well as in non-pregnant elderly patients particularly those with significant underlying diseases. The study was carried out to determine the clinical spectrum, antibiogram and outcome of Group B Streptococcus infection as it can cause invasive infections in non-pregnant adults that can lead to substantial morbidity and mortality in them. Materials and Methods: A retrospective observational chart based study was conducted in a tertiary care centre for a period of one year (June 2016 to May 2017). Multisite samples like pus, wound swabs, blood, body fluids and urine samples were collected from male and female adult patients (adults were defined as >18 years old) with Group B streptococcus infections. The samples were processed for culture and sensitivity aerobically according to the standard operating guidelines. Statistical analysis of the data was performed using SPSS v.21 by frequency, percentage, Fisher’s exact test and a p value less than 0.5 was considered as significant. Results: In 156 cases of GBS infections among non-pregnant adults, the prevalence was higher in the age group of 50-64 years (37.8%) with female to male ratio of 1.1:1. The most frequent clinical presentations were urinary tract infections (65.4%) with a significant female gender predominance (p value <0.0001). All tested isolates were susceptible to Ampicillin, Cephalosporins, Penicillin and Vancomycin. Conclusion: Elderly people with underlying diseases are more prone to GBS infections. Therefore, evaluation of risk factors and development of an antibiotic policy is useful for the successful treatment of GBS infections. Keywords: Noninvasive; Non-Pregnant; Streptococcus Agalactiae; Group B Streptococcus.