Introduction: Burn patients are at a risk of infection because of their destroyed skin barrier, suppressed immune system compounded by prolonged hospital stay and invasive therapeutic & diagnostic procedure. Despite various advances in infection control measures like early detection of causative agent and use of newer and broad spectrum antibiotics, management of burn septicemia still remains a big challenge. Aim and Objective: To study prevalence of various aerobic bacterial isolates among burn wound infection and to study their antimicrobial susceptibility pattern. Material and Methods: During study period of Jan- June 2017, a total of 369 pus samples were received and inoculated on different media as per standard protocol. Isolate were identified and antimicrobial susceptibility testing was done as per CLSI guidelines (2016). Results and observation: Out of 369 samples, 241 (65.31%) samples were from female and 128 (34.69%) from male. Among these 209 (56.64%) samples were having single isolate and in 125 (33.88%) samples, two types of bacteria were isolated. Out of 459 isolates, 133 Gram positive cocci and 326 Gram negative bacilli were isolated. Among various bacterial isolates Pseudomonas aeruginosa was most commonly isolated bacteria followed by Staphylococcus spp. and Klebsiella pnemoniae. Most of the isolates were resistant to routinely used antimicrobial agents. Conclusion: P. aeruginosa and S. aureus are the leading cause of infection in burn patients and isolation of multidrug resistant organism should be considered as a serious risk in burn unit. Early identification of infection caused by multidrug resistant organisms might help to modify treatment and outcome in burn patient. Keywords: Burn Wound Infection; P. Aeruginosa; S. Aureus and Multidrug Resistance.