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Journal of Microbiology and Related Research

Volume  6, Issue 1, January-June 2020, Pages 15-25


Original Article

Bacteriological Profile and Antimicrobial Sensitivity Pattern of Blood Cultures in a Diagnostic Centre of Central Madhya Pradesh: A Retrospective Study
Sadhna Sodani1, Ranjana Hawaldar2
1,2Associate Professor, Department of Medical Microbiology, Mahatma Gandhi Memorial Medical College.
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Introduction: The blood is sterile in normal conditions but due to invasion of microorganisms,it can cause severe fulminant septicemia and even death. A blood culture showing bacterial growth in culture has a high positive predictive value and aids the clinician in providing an accurate diagnosis as well as initiating appropriate antimicrobial treatment. The present study was undertaken to study the prevalence of etiological pathogens of blood stream infections in patients coming to our diagnostic centre and to study the antibiotic sensitivity pattern of
various isolates. Materials and Methods:- This was a retrospective study carried out on 1715 patients of all ages and both sexes in the microbiology department of our diagnostic centre between January to December 2019. Samples were collected by following thorough aseptic techniques and a blood volume of 5-10 ml in adults and 2-3 ml in
paediatric patients was collected in blood culture bottles ( Biomerieux). Identification and Antibiotic sensitivity was done on Vitec II(Biomerieux) according to CLSI guidelines. Results:- There were 1024(59.7%)males and 691(40.3%) females with a male to female ratio of 1.48:1.Maximum patients (n=555)were neonates accounting for 32.36% of all patients.Bacterial growth was observed in 424 (24.7%) patients .No bacterial growth was observed in 1219(75.3%) patients . Coagulase positive Staph aureus was the most common isolate accounting for 38.44% of all bacterial isolates .Candida sps was the second most common microorganism (12.74% ) Colistin showed 100% sensitivity to all Gram negative bacteria while MDR was observed in Citrobacter, E.coli ,Pseudomonas and Acinetobacter. Among the Gram positive bacterial isolates, Teicoplanin ,Tigecycline and Liezolid demonstrated 100% sensitivity,whereas 66.6% bacteria were sensitive to clindamycin and Daptomycin. 100% resistance was observed to Erythromycin, Oxacillin and Trimethoprim/sulfamethaxazole. Conclusion: Our study provides an insight into the prevalence of bacterial pathogens causing blood stream infections in Central Madhya Pradesh region as well as the antibiotic susceptibility pattern of these bacteria. Restricted use of antibiotics, use of a combination of antibiotics will help in reducing the development of resistance to common antibiotics.

Keywords: Lood Stream Infections; Antibiotic Sensitivity; Blood Culture; MDR .

Corresponding Author : Sadhna Sodni