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Journal of Microbiology and Related Research

Volume  6, Issue 1, January-June 2020, Pages 9-13


Original Article

Comparison of Ziehl-Neelsen Staining, Fluorescent Staining and PCR in the Diagnosis of Pulmonary Tuberculosis
Shwetha D C1, Usha M G2
1,Assistant Professor, Department of Microbiology, Adichunchanagiri Institute of Medical Sciences, B G Nagara Nagamangala Taluk, Mandya, 2 Professor, Department of Microbiology, Jagadguru Jayadeva Murugarajendra Medical College, Davangere.
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Introduction: Tuberculosis remains one of the deadliest diseases in the world. The traditional bacterial diagnostic methods are either slow or their sensitivity is very low. So, there is a need for rapid accurate diagnostic test like PCR (Polymerase Chain Reaction). Materials and Methods: 100 sputum samples were collected from 50 clinically suspected cases of Pulmonary Tuberculosis as per RNTCP guidelines. Each sputum sample was subjected to ZN (Ziehl-Neelsen) staining, fluorescent staining and PCR. The sputum sample found to be negative by direct smear was then subjected to concentration technique by NALC-NaOH (N-acetyl-L-cysteine-sodium hydroxide) method and staining methods were repeated. Results: Out of 100 sputum samples examined, 44 (44%) were positive and 56 (56%) were negative by ZN staining. 52 (52%) were positive and 48 (48%) were negative by fluorescent staining and 76(76%) samples were positive by PCR. Conclusion: The rapidity and more positivity rate in PCR for the diagnosis of Tuberculosis,encourages the routine use of this in clinical practices.

Keywords: ZN staining; Fluorescent staining; PCR; Tuberculosis.

Corresponding Author : Shwetha D C