Background: Asphyxia is the condition of increased PCO2 and decreased PO2 in blood. It is of general opinion that carbondioxide excess and oxygen lack stimulate the hypothalamus and as a result activate the sympathetic system causing sympatho-adrenal discharge  has described that the activation of the sympathy-oadrenal system depends on an intact brain stem and spinal cord, although there is probably some direct action of CO2 on the adrenal medulla. Thus, the effect of asphyxia on the hypothalamus and medullary vasomotor centre directly or indirectly through chemo-receptors is the increased systemic pressor response and renal functions due to increased sympatho-adrenal activity. Aims and Objective: In the present study, try to find out the role of spinal centre in asphyxia and asphyxia with nicotine (drip) condition at the time of hypertension and urine flow. Methods: Experimental asphyxia (40 to 90 seconds) was done after tracheostomy and nicotine drip (8–10 drop/min) used intravenously through femoral vein. The spinal cord was opened by laminectomy at the level of C7–C8. Both ends of the opened spinal cord was tied by cotton thread and then sectioned when needed. Results: In such spinal animals asphyxia failed to alter the blood pressures both in control and nicotinised animals. But the changes in urine flow in such animals; the diuresis is partially counteracted whereas the antidiuresis is slightly but significantly augmented in comparison to the control. Conclusion: Therefore, from the above observations of the present study, it may be argued that asphyxia and asphyxia with nicotine (drip) act through supraspinal centers for the alterations in blood pressure and urine flow.