Background: A parallel design clinical study evaluated reduction in dentin hypersensitivity after brushing for 6 weeks with Sensodyne Rapid relief toothpaste (containing sodium fluride and strontium acetate), Dabur Hypersensitive toothpaste (containing strontium acetate and herbal extracts), Dabur Red toothpaste- with akarkara and Dabur Red toothpaste (containing herbal extracts). Objective: To evaluate and compare the efficacy of a dentifrice containing sodium fluride and strontium acetate, strontium acetate (in herbal base) and two herbal dentifrices in reducing dentinal hypersensitivity. Materials and Methods: 139  outdoor patients  (83 males, 56 females) in 20-60 years age group (mean age =  33.36 + 9.87 years); attending the OPD at Inderprastha Dental College and Hospital, Sahibabad - Ghaziabad, India; made up the study population. Subjects identified with two hypersensitive teeth using the tactile and air blast (Schiff’s Scale) hypersensitivity methods were assigned to their treatment group. Dentin hypersensitivity and oral tissues were evaluated at baseline, immediately after the single application, 3 weeks and after 6 weeks. Results: The 6 week scores as compared to baseline scores for tactile and air blast tests in the two groups showed a reduction in tooth hypersensitivity. Test Product A (Sensodyne Rapid relief toothpaste) was found to reduce Tactile sensitivity better than Test Product B (Dabur Hyper sensitive toothpaste) and much better than Test Product C (Dabur Red Tooth Paste with akarkara) and D (Dabur Red Tooth Paste). But Test Product B was found to reduce Evaporative air blast sensitivity slightly better than Test Product A and much better than Test Product C, and D. Instant relief from dentin hypersensitivity was reported on single application with test products A (Sensodyne Rapid relief toothpaste) and B (Dabur Hyper sensitive toothpaste) but not with products C (Dabur Red Tooth Paste with akarkara) and D (Dabur Red Tooth Paste). Conclusion: Two of the four products reduced dentinal hypersensitivity in the study subjects at the end of the 6-week period. However, there was no statistically significant difference in reduction in hypersensitivity between these two products.