Bestiality or animal sexual abuse (ASA) is a type of unnatural sexual offense in which animals are used as a medium for satisfying sexual desire without developing any kind of emotional bonding. With the development of the DNA fingerprinting methods (restriction fragment length polymorphism), Dr. Alec Jeffreys provided the first molecular genetic tools to genetically differentiate between humans in the late 20th century, which initiated the replacement of protein-based methods in forensic testing and has revolutionized forensic investigations and it became a very important tool in criminal cases as well as civil cases. DNA profiling is golden technology in the investigation of rape cases as well as bestiality. In this case, a video had become viral in which a person was wearing a white shirt, he was having unnatural sex with a red color cow. During the police investigation, on the basis of viral video and thread, the police reached the suspect and the suspect accepted all his crimes in front of the police. The forensic samples (Vaginal swab of cow, underwear of suspect) and reference sample (blood sample of the suspect on FTA card) related to the case were received. Human semen was detected on the vaginal swab of the cow and the underwear of the suspect. After DNA examination, the same male DNA profile was obtained from the reference blood sample of the suspect and underwear of the suspect which is matching with the male DNA profile obtained from the source of the vaginal mucous swab of Cow