Introduction:Endotracheal suctioning (ETS) is one of the most common procedures performed in patients with artificial airways. It is a component of bronchial hygiene therapy and mechanical ventilation that involves the mechanical aspiration of pulmonary secretions from a patient’s artificial airway to prevent its obstruction. The procedure includes patient preparation, the suctioning event, post procedure care. The objectives of the study were to (1) To observe the existing practice regarding endotracheal suctioning among staff nurses. (2) To evaluate practice regarding endotracheal suctioning among staff nurses after demonstration. (3) To find out association of practice with their selected demographic variables. Method: One group pretest and post-test pre-experimental study design by using structured observation checklist to assess practice of endotracheal suctioning among staff nurses. The study was conducted among 30 staff nurses working in ICU of selected hospital by using non probability convenient sampling technique. The data was collected and analyzed based on objectives of the study using descriptive and inferential statistics. Result: The overall comparison of pre-test practice score and post-test practice score of staff nurses regarding endotracheal suctioning mean of pre-test practice score was 7.57 and post-test practice score was 12.17. The tabulated ‘t’ value was 1.69 and the calculated ‘t’ value was 13.73 are much higher than the tabulated value at p<0.05 level of significance for overall practice score of subjects which was statistically acceptable level of significance. Hence it was statistically interpreted that the demonstration was an effective tool in improving the practice regarding endotracheal suctioning among subjects. Conclusion: The investigator concludes that the demonstration was effective and will help to improve the practice of staff nurses.